Publications

Distribution and diversity of classical deacylases in bacteria.
Graf LG, Moreno-Yruela C, Qin C, Schulze S, Palm GJ, Schmöker O, Wang N, Hocking DM, Jebeli L, Girbardt B, Berndt L, Dörre B, Weis DM, Janetzky M, Albrecht D, Zühlke D, Sievers S, Strugnell RA, Olsen CA, Hofmann K, Lammers M. Nat Commun. 2024 Nov 3;15(1):9496. doi: 10.1038/s41467-024-53903-0.

Monoclonal antibody neutralizes Staphylococcus aureus serine protease-like protein B (SplB)-induced pathology.
Iqbal J, von Fournier J, Wittmann N, Darisipudi MN, Mrochen DM, Smiljanov B, Surmann K, Wockenfuß G, Steil L, Kohler TP, Glinka FL, Peringathara S, Saade C, Fernandes LM, Bornscheuer U, Reichel CA, Bröker BM, Raafat D, Holtfreter S. Infect Immun. 2025 Jun 13:e0017125. doi: 10.1128/iai.00171-25

Microenvironments of tuberculous granuloma: advances and opportunities for therapy.
Krueger G, Faisal S, Dorhoi A. Front Immunol. 2025 Mar 24;16:1575133. doi: 10.3389/fimmu.2025.1575133. eCollection 2025.

Functional and Proteomic Dissection of the Contributions of CodY, SigB and the Hibernation Promoting Factor HPF to Interactions of Staphylococcus aureus USA300 with Human Lung Epithelial Cells.
Li X, Busch LM, Piersma S, Wang M, Liu L, Gesell Salazar M, Surmann K, Mäder U, Völker U, Buist G, van Dijl JM.J Proteome Res. 2024 Oct 4;23(10):4742-4760. doi: 10.1021/acs.jproteome.4c00724.

The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in Streptococcus pneumoniae.
Mebus VH, De S, Busch LM, Gesell Salazar M, Schlüter R, Völker U, Hammerschmidt S, Frees D. Microbiol Spectr. 2025 May 23:e0080425. doi: 10.1128/spectrum.00804-25. 

Improved recombinant expression of soluble cathepsin B and L in Escherichia coli.
Möller C, Rimkus N, Skala FFO, Merouze M, Böttcher D, Dörr M, Bornscheuer UT. Appl Microbiol Biotechnol. 2024 Dec 16;108(1):536. doi: 10.1007/s00253-024-13374-1.

Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O.
Möller C, Terholsen H, Schmöker O, Lê TLA, Wesche J, Schmiade P, Eppendorfer E, Rimkus N, Girbardt B, Böttcher D, Palm GJ, Hoppen J, Lammers M, Greinacher A, Aurich K, Bornscheuer UT. Chembiochem. 2025 Apr 1;26(7):e202500072. doi: 10.1002/cbic.202500072.

DDI2 protease controls embryonic development and inflammation via TCF11/NRF1.
Nedomova M, Haberecht-Müller S, Möller S, Venz S, Prochazkova M, Prochazka J, Sedlak F, Chawengsaksophak K, Hammer E, Kasparek P, Adamek M, Sedlacek R, Konvalinka J, Krüger E, Grantz Saskova K .iScience. 2024 Sep 5;27(10):110893. doi: 10.1016/j.isci.2024.110893.

Activating the NFE2L1-ubiquitin-proteasome system by DDI2 protects from ferroptosis.
Ofoghi A, Kotschi S, Lemmer IL, Haas DT, Willemsen N, Bayer B, Jung AS, Möller S, Haberecht-Müller S, Krüger E, Krahmer N, Bartelt A.Cell Death Differ. 2025 Mar;32(3):480-487. doi: 10.1038/s41418-024-01398-z.

MassSpecPreppy-An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications.
Reder A, Hentschker C, Steil L, Gesell Salazar M, Hammer E, Dhople VM, Sura T, Lissner U, Wolfgramm H, Dittmar D, Harms M, Surmann K, Völker U, Michalik S. Proteomics. 2024 May;24(9):e2300294. doi: 10.1002/pmic.202300294.

pTripleTREP - A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus.
Wolfgramm H, Saade C, Harms M, Busch LM, Lange J, Schedlowski M, Surmann K, Gesell Salazar M, Hentschker C, Steil L, Michalik S, Völker U, Reder A. Microb Cell Fact. 2025 May 20;24(1):115. doi: 10.1186/s12934-025-02736-7.

Linking Virulence and Iron Limitation Response in Staphylococcus aureus: The sRNA IsrR Is Involved in SaeRS Activation.
Busch LM, Ganske A, Reißel S, Bleul L, Hentschker C, Wolfgramm H, Steil L, Gesell Salazar M, Schaffer M, Reder A, Michalik S, Wolz C, Surmann K, Völker U, Mäder U.J Proteome Res. 2025 Jun 2. doi: 10.1021/acs.jproteome.5c00059. 

Two RNA Folds from One Sequence: A Ribozyme with Versatile Substrate Processing Abilities.
Zhu J, Dierks D, Möller C, Balke D, Müller S.Angew Chem Int Ed Engl. 2024 Sep 9;63(37):e202409047. doi: 10.1002/anie.202409047.

Biochemical analyses of cystatin-C dimers and cathepsin-B reveals a trypsin-driven feedback mechanism in acute pancreatitis
Modenbach, J.M., Möller, C., Asgarbeik, S., Geist, N., Rimkus, N., Dörr, M., Wolfgramm, H., Steil, L., Susemihl, A., Graf, L., Schmöker, O., Böttcher, D., Hammer, E., Glaubitz, J., Lammers, M., Delcea, M., Völker, U., Aghdassi, A.A., Lerch, M.M., Weiss, F.U., Bornscheuer, U.T., Sendler, M. (2025), Nat. Commun., 16, 1702

Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases
Glinka, F.L., Schmöker, O., Singh, A.K., Steil, L., Hentschker, C., Völker, U., Böttcher, D., Lammers, M., Cammann, C., Seifert, U., Krüger, E., Naumann, M., Bröker, B.M., Bornscheuer, U.T. (2025), AMB Express, DOI: 10.1186/s13568-025-01841-5

Improved recombinant expression of soluble cathepsin B and L in Escherichia coli
Christina Möller, Niklas Rimkus, Ferdinand F. O. Skala, Maëlle Merouze, Dominique Böttcher, Mark Dörr, Uwe T. Bornscheuer
Biotechnologically Relevant Enzymes and Proteins, Volume 108, article number 536, (2024)

PSMD11 loss-of-function variants correlate with a neurobehavioral phenotype, obesity, and increased interferon response
Wallid Deb , Cory Rosenfelt , Virginie Vignard , Jonas Johannes Papendorf , Sophie Möller , Martin Wendlandt , Maja Studencka-Turski , Benjamin Cogné , Thomas Besnard , Léa Ruffier , Bérénice Toutain , Léa Poirier , Silvestre Cuinat , Amy Kritzer , Amy Crunk , Janette diMonda , Jaime Vengoechea , Sandra Mercier , Lotte Kleinendorst , Mieke M. van Haelst, Linda Zuurbier , Telma Sulem , Hildigunnur Katrínardóttir , Rún Friðriksdóttir , Patrick Sulem , Kari Stefansson , Berglind Jonsdottir , Shimriet Zeidler , Margje Sinnema , Alexander P.A. Stegmann , Natali Naveh , Cara M. Skraban , Christopher Gray , Jill R. Murrell , Sedat Isikay , Davut Pehlivan , Daniel G. Calame , Jennifer E. Posey , Mathilde Nizon , Kirsty McWalter , James R. Lupski , Bertrand Isidor , François V. Bolduc , Stéphane Bézieau , Elke Krüger , Sébastien Küry , Frédéric Ebstein
The American Journal of Human Genetics, Volume 111, Issue 7, 11 July 2024, Pages 1352-1369, doi: 10.1016/j.ajhg.2024.05.016

DDI2 protease controls embryonic development and inflammation via TCF11/NRF1
Monika Nedomova , Stefanie HaberechtMüller , Sophie Möller , Simone Venz , Michaela Prochazkova , Jan Prochazka , Frantisek Sedlak , Kallayanee Chawengsaksophak, Elke Hammer , Petr Kasparek , Michael Adamek, Radislav Sedlacek , Jan Konvalinka , Elke Krüger , Klara Grantz Saskova
iScience, Volume 27, Issue 10, 18 October 2024, 110893. doi: 10.1016/j.isci.2024.110893

Biallelic USP14 variants cause a syndromic neurodevelopmental disorder.
Ebstein F, Latypova X, Hung KYS, Prado MA, Lee BH, Möller S, Wendlandt M, Zieba BA, Florenceau L, Vignard V, Poirier L, Toutain B, Moroni I, Dubucs C, Chassaing N, Horvath J, Prokisch H, Küry S, Bézieau S, Paulo JA, Finley D, Krüger E, Ghezzi D, Isidor B.
Genet Med. 2024 Mar 9:101120. doi: 10.1016/j.gim.2024.101120. Online ahead of print.PMID: 38469793 

Identification of eight novel proteasome variants in five unrelated cases of proteasome-associated autoinflammatory syndromes (PRAAS).
Papendorf JJ, Ebstein F, Alehashemi S, Piotto DGP, Kozlova A, Terreri MT, Shcherbina A, Rastegar A, Rodrigues M, Pereira R, Park S, Lin B, Uss K, Möller S, da Silva Pina AF, Sztajnbok F, Torreggiani S, Niemela J, Stoddard J, Rosenzweig SD, Oler AJ, McNinch C, de Guzman MM, Fonseca A, Micheloni N, Fraga MM, Perazzio SF, Goldbach-Mansky R, de Jesus AA, Krüger E.
Front Immunol. 2023 Aug 4;14:1190104. doi: 10.3389/fimmu.2023.1190104. eCollection 2023.

Immune response mechanisms in acute and chronic pancreatitis: strategies for therapeutic intervention.
Glaubitz J. Asgarbeik S. Lange R. Mazloum H. Elsheikh H. Weiss F.U. Sendler M.
Front Immunol. 2023 Oct 10;14:1279539.

IRAK3-mediated suppression of pro-inflammatory MyD88/IRAK signaling affects disease severity in acute pancreatitis.
Thiel FG, Asgarbeik S, Glaubitz J, Wilden A, Lerch MM, Weiss FU, Sendler M.
Sci Rep. 2023 Jul 4;13(1):10833. doi: 10.1038/s41598-023-37930-3.

Establishing 20S Proteasome Genetic, Translational and Post-Translational Status from Precious Biological and Patient Samples with Top-Down MS
Sanchez Dafun A, Živković D, Adonai Leon-Icaza, Möller S, Froment C, Bonnet D, Almeida de Jesus A, Alric L, Quaranta-Nicaise M, Ferrand A, Cougoule C, Meunier E, Burlet-Schiltz O, Ebstein F, Goldbach-Mansky R, Krüger E, Bousquet MP , Marcoux J
Cells 2023, 12(6), 844; doi: 10.3390/cells12060844.

Activated regulatory T-cells promote duodenal bacterial translocation into necrotic areas in severe acute pancreatitis
Glaubitz J, Wilden A, Frost F, Ameling S, Homuth G, Mazloum H, Rühlemann MC, Bang C, Aghdassi AA, Budde C, Pickartz T, Franke A, Bröker BM, Voelker U, Mayerle J, Lerch MM, Weiss FU, Sendler M.
Gut. 2023 Jan 11:gutjnl-2022-327448. doi: 10.1136/gutjnl-2022-327448. Online ahead of print. PMID: 36631247

In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response
Glaubitz J, Wilden A, Golchert J, Homuth G, Völker U, Bröker BM, Thiele T, Lerch MM, Mayerle J, Aghdassi AA, Weiss FU, Sendler M.
Nat Commun. 2022 Aug 3;13(1):4502. doi: 10.1038/s41467-022-32195-2., PMID: 35922425

Pneumococcal Extracellular Serine Proteases: Molecular Analysis and Impact on Colonization and Disease.
Ali MQ, Kohler TP, Schulig L, Burchhardt G, Hammerschmidt S.
Front Cell Infect Microbiol. 2021 Nov 1;11:763152. doi: 10.3389/fcimb.2021.763152. eCollection 2021.PMID: 34790590 

Mobilization of CD11b+/Ly6chi monocytes causes multi organ dysfunction syndrome in acute pancreatitis.
Wilden A, Glaubitz J, Otto O, Biedenweg D, Nauck M, Mack M, Ribback S, Bröker BM, von Rheinbaben SF, Lerch MM, Aghdassi AA, Weiss FU, Sendler M.
Front Immunol. 2022 Oct 10;13:991295. doi: 10.3389/fimmu.2022.991295. eCollection 2022.PMID: 36300116